THERMO T4 DNA連接酶,T4 DNA Ligase
描述
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
T4 DNA Ligase requiresATPas a cofactor.
Highlights
• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast—sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligation
THERMO T4 DNA連接酶,T4 DNA Ligase
訂購信息:
| 貨號 | 單位 | |
|---|
| EL0011 | 1,000 units | | |
| EL0012 | 5 x 1,000 units | | |
| EL0014 | 200 units | |
THERMO T4 DNA連接酶,T4 DNA Ligase
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