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雙染蛋白MARKERPrecision Plus Protein™ Dual Color StandPrecision Plus Protein™ Dual Color Standards #161-0374

500 μl, mixture of 10 recombinant proteins （10–250 kD）, 8 blue-stained bands, and 2 pink reference bands （25 and 75 kD）, 50 applications

Description技術(shù)指標(biāo)

MW size range, kD	10–250
Stain type	Dual Color
Volume, µl	500
Total number of applications	50
Shelf life at –20°C, months	12
Loading volume on mini gels, µl	for Coomassie staining, 10
Loading volume on mini gels, µl	for fluorescent blot detection, 5 of 1:10 dilution
Application Type
Monitoring electrophoretic separation for SDS-PAGE gels	Yes
Molecular weight sizing on SDS-PAGE gels	Yes
Monitoring blot transfer efficiency	Yes
Fluorescence blots	Yes
Molecular weight sizing on	N/A
Loading Volume on Mini Gels
For Coomassie staining, µl	N/A
For stain-free gels, µl	N/A
For silver staining, µl	N/A
For SYPRO Ruby staining, µl	N/A
For blot development, µl	N/A
StrepTactin conjugate working dilutions for colorimetric blot development	N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development	N/A

The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins （10–250 kD）, including eight blue-stained bands and two pink reference bands （25 and 75 kD）.

雙染蛋白MARKERPrecision Plus Protein™ Dual Color Stand

Applications and Uses

Choose Precision Plus Protein Dual Color standards for SDS-PAGE:

Monitoring electrophoretic separationMolecular weight sizingMonitoring blot transfer efficiency with easy orientation via multicolor standardsFluorescent blot detection, with blue bands visualized in the red wavelength range and pink bands in the green wavelength （prepare a 1:10 dilution of the Dual Color standard and load 5 µl per well）

More Information

Loading buffer:30% （w/v） glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN₃, 0.01% bromophenol blue