人妻一区二区中文字幕在线-大肉棒抽插小嫩穴视频免费-国产福利萌白酱视频资源福利-最新国产主播一区二区-伊人五月天婷婷成人-色av中文字幕在线-美女打开小穴让男生操的视频-国产又长又粗又长又爽毛片-人人妻人人澡人人爽欧美三区

fermentas逆轉(zhuǎn)錄試劑盒RevertAid First Strand cDNA Synthesis Kit說明書

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit是一套用于以RNA為模板高效合成*鏈cDNA的完整系統(tǒng)，可合成長達(dá)13kb的cDNA。試劑盒配套提供的RiboLockRNase Inhibitor，可有效防止RNA模板的降解。試劑盒配套提供oligo(dT)18引物和隨機(jī)六聚體引物。oligo(dT)18引物可以選擇性地與mRNA中的poly (A)尾結(jié)合。隨機(jī)六聚體引物無需poly (A)尾，因此可轉(zhuǎn)錄mRNA 5’-端區(qū)域，也可以無poly (A)尾的RNA (如micoRNA)為模板合成cDNA。該試劑盒也可使用基因特異性引物。優(yōu)點(diǎn)：

應(yīng)用:

fermentas逆轉(zhuǎn)錄試劑盒RevertAid First Strand cDNA Synthesis Kit

RevertAid First Strand cDNA Synthesis KitFeatures·Full-lengthfirst strand cDNA up to 13 kb.·Increased reaction temperaturesin the range of 42-50°C.·Supplied withthe recombinant RiboLock?RNase Inhibitor.·Complete–oligo(dT)18and random hexamer primers included with the kit.Applications·First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2).·Construction of full length cDNA libraries.·Generation of probes for hybridization.·aRNA synthesis.DescriptionThe RevertAid?First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb.The kit uses RevertAid?Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase.The recombinant RiboLock?RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo(dT)18and random hexamer primers. The oligo(dT)18anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.The first strand of cDNA can be directly used as a template in PCR (seeRT-PCR protocol), real-time PCR or in second strand cDNA synthesis (seeprotocol).Quality ControlThe kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.

is Kit

RevertAid First Strand cDNA Synthesis Kit合成全長cDNA1 μg小鼠心臟總RNA作為模板，用oligo(dT)18 引物進(jìn)行逆轉(zhuǎn)錄反應(yīng)。由此合成的cDNA，再作為Taq DNA Polymerase后續(xù)PCR擴(kuò)增的模板。末端2024 bp片段的成功擴(kuò)增，表明長度為13.8 kb的全長RNA片段得到成功的逆轉(zhuǎn)錄。產(chǎn)品組成RevertAid First Strand cDNA Synthesis Kit包含RevertAid Reverse Transcriptase、RiboLock RNase Inhibitor、5x Reaction Buffer、dNTP Mix、Oligo(dT)18 引物、隨機(jī)六聚體引物、對照GAPDH RNA、10 μM 正向GAPDH引物、10 μM反向GAPDH 引物以及nuclease-free water。