LIFE ambion PureLink RNA Mini Kit（貨號12183025）
Ambion公司成立于1988年，從1989年開始在美國德克薩斯州的奧斯丁市開始運(yùn)營。公司在劍橋和日本都設(shè)有分支機(jī)構(gòu)。Ambion本身稱為RNA公司自有其道理，自成立以來它所有產(chǎn)品都圍繞RNA的。在RNAi這方面，他們從2001年開始提供相關(guān)產(chǎn)品，是zui早提供RNAi產(chǎn)品公司之一。
目前Ambion公司提供六種RNAi產(chǎn)品，它們分別是：化學(xué)合成、利用試劑盒轉(zhuǎn)錄獲得、利用RNA酶將長片段雙鏈RNA切割獲得、利用質(zhì)粒表達(dá)shRNA、利用PCR產(chǎn)物表達(dá)shRNA和從病毒載體表達(dá)shRNA。除了siRNA外，公司還提供將100個到1000個siRNA導(dǎo)入細(xì)胞的轉(zhuǎn)染和電穿孔產(chǎn)品。
美國Ambion是一家聞名 的RNA公司，自2001年起Ambion開始提供RNAi及其相關(guān)產(chǎn)品，經(jīng)過幾年努力，今年Ambion被Science評為zui高技術(shù)的RNAi技術(shù)公司。
LIFE ambion PureLink RNA Mini Kit（貨號12183025）
產(chǎn)品簡介：
描述
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications
Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.
For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.
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