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產(chǎn)品介紹Mouse IgG1 Negative Control antibodyis suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES產(chǎn)品詳情

Target Species

Negative Control

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Immunogen

Human T lymphocytes.

Approx. Protein ConcentrationsIgG concentration 1.0 mg/mlEndotoxin Level<0.01 EU/ug

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存儲(chǔ)條件Store at -20oC only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.應(yīng)用Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

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This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells in 100ul文獻(xiàn)1. Weiss, D.J.et al.(2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.avium.J Leukoc Biol. 83 (1): 48-55.2. Chen, W.et al.(2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.Mol Vis. 15: 619-28.3. Safeukui Iet al.(2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.MBio. 6 (1) pii: e02493-14.4. Aricha, R.et al.(2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.J Autoimmun. 67: 57-64.5. Wattegedera, S.R.et al.(2017) Enhancing the toolbox to study IL-17A in cattle and sheep.Vet Res. 48 (1): 20.6. Stangl, H.et al.(2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.Sci Rep. 10 (1): 17071.